Open AccessImprovement of axial resolution and contrast in temporally focused widefield two-photon microscopy with structured light illumination
Author(s) -
Heejin Choi,
Elijah Y. S. Yew,
Bertan Hallacoglu,
Sergio Fantini,
Colin J. R. Sheppard,
Peter T. C. So
Publication year - 2013
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.4.000995
Subject(s) - optics , microscopy , photon , resolution (logic) , point spread function , image resolution , two photon excitation microscopy , near field scanning optical microscope , materials science , physics , light scattering , light sheet fluorescence microscopy , scattering , optical microscope , scanning confocal electron microscopy , computer science , scanning electron microscope , fluorescence , artificial intelligence
Although temporally focused wide-field two-photon microscopy (TFM) can perform depth resolved wide field imaging, it cannot avoid the image degradation due to scattering of excitation and emission photons when imaging in a turbid medium. Further, its axial resolution is inferior to standard point-scanning two-photon microscopy. We implemented a structured light illumination for TFM and have shown that it can effectively reject the out-of-focus scattered emission photons improving image contrast. Further, the depth resolution of the improved system is dictated by the spatial frequency of the structure light with the potential of attaining depth resolution better than point-scanning two-photon microscopy.
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