Laser-assisted optoporation of cells and tissues – a mini-review | Zendy

Herbert Schneckenburger | Zendy

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Laser-assisted optoporation of cells and tissues – a mini-review

Author(s) -

Herbert Schneckenburger

Publication year - 2019

Publication title -

biomedical optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.362

H-Index - 86

ISSN - 2156-7085

DOI - 10.1364/boe.10.002883

Subject(s) - microbeam , photothermal therapy , laser , optics , materials science , transfection , fluorescence , continuous wave , optoelectronics , biophysics , dye laser , nanotechnology , cell culture , biology , physics , genetics

Laser microbeam techniques are presented, which permit the introduction of molecules or small particles into living cells. Possible mechanisms - including photochemical, photothermal and opto-mechanical interactions (ablations) - are induced by continuous wave (cw) or pulsed lasers of different wavelength, power, and mode of operation. Laser-assisted optoporation permits the uptake of fluorescent dyes as well as DNA plasmids for cell transfection, and, in addition to its broad application to cultivated cells, may have some clinical potential.

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