Parallel on-axis holographic phase microscopy of biological cells and unicellular microorganism dynamics | Zendy

Natan T. Shaked | Zendy; Thomas M. Newpher | Zendy; Michael Ehlers | Zendy; Adam Wax | Zendy

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Parallel on-axis holographic phase microscopy of biological cells and unicellular microorganism dynamics

Author(s) -

Natan T. Shaked,

Thomas M. Newpher,

Michael Ehlers,

Adam Wax

Publication year - 2010

Publication title -

applied optics

Language(s) - English

Resource type - Journals

ISSN - 0003-6935

DOI - 10.1364/ao.49.002872

Subject(s) - holography , digital holographic microscopy , optics , frame rate , microscopy , holographic interferometry , interferometry , digital holography , bandwidth (computing) , spatial light modulator , light sheet fluorescence microscopy , computer science , spatial frequency , phase (matter) , biological system , physics , scanning confocal electron microscopy , biology , computer network , quantum mechanics

We apply a wide-field quantitative phase microscopy technique based on parallel two-step phase-shifting on-axis interferometry to visualize live biological cells and microorganism dynamics. The parallel on-axis holographic approach is more efficient with camera spatial bandwidth consumption compared to previous off-axis approaches and thus can capture finer sample spatial details, given a limited spatial bandwidth of a specific digital camera. Additionally, due to the parallel acquisition mechanism, the approach is suitable for visualizing rapid dynamic processes, permitting an interferometric acquisition rate equal to the camera frame rate. The method is demonstrated experimentally through phase microscopy of neurons and unicellular microorganisms.

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