Towards the clinical application of laser-induced breakdown spectroscopy for rapid pathogen diagnosis: the effect of mixed cultures and sample dilution on bacterial identification | Zendy

Steven J. Rehse | Zendy; Qassem I. Mohaidat | Zendy; Sunil Palchaudhuri | Zendy

Open Access

Towards the clinical application of laser-induced breakdown spectroscopy for rapid pathogen diagnosis: the effect of mixed cultures and sample dilution on bacterial identification

Author(s) -

Steven J. Rehse,

Qassem I. Mohaidat,

Sunil Palchaudhuri

Publication year - 2010

Publication title -

applied optics

Language(s) - English

Resource type - Journals

ISSN - 0003-6935

DOI - 10.1364/ao.49.000c27

Subject(s) - laser induced breakdown spectroscopy , spectroscopy , dilution , materials science , pathogen , laser , analytical chemistry (journal) , optics , microbiology and biotechnology , biology , chemistry , chromatography , physics , quantum mechanics , thermodynamics

Laser-induced breakdown spectroscopy has been utilized to classify and identify bacterial specimens on the basis of their atomic composition. We have characterized the effect that the presence of a second bacterial species in the ablated specimen had on the identification of the majority species. Specimens with a reduced number of bacterial cells (approximately 2500) were identified with 100% accuracy when compared to undiluted specimens. In addition, a linear dependence of the total spectral power as a function of cell number was determined. Lastly, a high selectivity was obtained for a LIBS-based analysis of nine separate bacterial strains from four genera.

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