Fluorescence preselection of bioaerosol for single-particle mass spectrometry
Author(s) -
M.A. Stowers,
A.L. van Wuijckhuijse,
J.C.M. Marijnissen,
Ch.E. Kientz,
Tomasz Ciach
Publication year - 2006
Publication title -
applied optics
Language(s) - English
Resource type - Journals
ISSN - 0003-6935
DOI - 10.1364/ao.45.008531
Subject(s) - bioaerosol , aerosol , mass spectrometry , fluorescence , particle (ecology) , particle beam , optics , materials science , particle size , beam (structure) , fluorescence spectrometry , laser induced fluorescence , analytical chemistry (journal) , laser , chemistry , physics , chromatography , oceanography , organic chemistry , geology
We have designed, constructed, and tested a system that pre-selects the biological fraction of airborne particles from the overall aerosol. The preselection is based on fluorescence emission excited by a continuous 266 nm laser beam. This beam is one of two cw beams used to measure the aerodynamic particle size of sampled particles. The intention in our system is that single particles, based on size and fluorescence emission, can be selected and further examined for chemical composition by mass spectrometry.
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