Open AccessImmunohistochemical Detection of Tumor Suppressor Gene p53 Protein in Feline Injection Site-associated Sarcomas
Author(s) -
Prashant R. Nambiar,
Marion L. Jackson,
John Ellis,
Brian J. Chelack,
Beverly A. Kidney,
Deborah M. Haines
Publication year - 2001
Publication title -
veterinary pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.794
H-Index - 89
eISSN - 1544-2217
pISSN - 0300-9858
DOI - 10.1354/vp.38-2-236
Subject(s) - immunohistochemistry , tumor suppressor gene , carcinogenesis , pathology , biology , p53 protein , gene , gene mutation , mutation , cancer research , medicine , genetics
Sarcomas associated with injection sites are a rare but important problem in cats. Immunohistochemical detection of p53 protein may correlate to mutation of the p53 tumor suppressor gene, a gene known to be important in oncogenesis. The expression of nuclear p53 protein in 40 feline injection site-assocated sarcomas was examined by immunohistochemical staining. In 42.5% (17/40), tumor cell nuclei were stained darkly; in 20% (8/40), tumor cell nuclei were stained palely; and in 37.5% (15/40), tumor cell nuclei were unstained. Immunohistochemical detection of p53 protein in a proportion of injection site-associated sarcomas suggests that mutation of the p53 gene may play a role in the pathogenesis of these tumors.