The Determination of Antibiotic Resistance of Helicobacter Pylori Isolated from Patients Living in North of Iran (Sari) | Zendy

Mojtaba Moradi Golrokhi | Zendy; Hafez Fakheri | Zendy; Mohammad Reza Haghshenas | Zendy; Mohammad Ahanjan | Zendy

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The Determination of Antibiotic Resistance of Helicobacter Pylori Isolated from Patients Living in North of Iran (Sari)

Author(s) -

Mojtaba Moradi Golrokhi,

Hafez Fakheri,

Mohammad Reza Haghshenas,

Mohammad Ahanjan

Publication year - 2016

Publication title -

universal journal of microbiology research

Language(s) - English

Resource type - Journals

eISSN - 2331-6721

pISSN - 2331-673X

DOI - 10.13189/ujmr.2016.040102

Subject(s) - resazurin , antimicrobial , broth microdilution , antibiotic resistance , traditional medicine , antibiotics , agar diffusion test , staphylococcus aureus , chemistry , microbiology and biotechnology , biology , food science , minimum inhibitory concentration , chromatography , bacteria , medicine , genetics

The present study was carried out to compare the antimicrobial activities of methanol leaf extracts of Bauhinia purpurea, Dicranopterislinearis, Melastomamalabathricum and Muntingiacalabura portrayed by different antimicrobial assays against methicillin-susceptible and methicillin- resistant Staphylococcus aureus strains. Antimicrobial activities of the methanol leaf extracts were preliminarily screened by disc diffusion. Minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) were determined by broth microdilution and colorimetric assay (resazurin). Based on disc diffusion method, S. aureusATCC®700699™ (MRSA) elucidated higher susceptibility pattern against all plant extracts compared to S. aureusATCC®25923™ (MSSA). Taking results from all employed assays into consideration, M. calaburamethanol leaf extract comparably elicited the highest antimicrobial activity than the other methanol leaf extracts against both microorganisms. The MIC values were determined by colorimetric assay (resazurin) due to pigmentations of the methanol leaf extracts that obscured visual growth turbidity inspection. Complication in colour changes observation in colorimetric assay to determine MBC was overcome by employing the conventional plating method. This study suggested that all antimicrobial assays should be carried out concurrently so as the data obtained can be comparatively analysed for a better outcome as each antimicrobial assay has its own shortfall.

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