Open AccessDevelopment of a 16S rRNA PCR-RFLP Assay for Bartonella identification: Applicability in the Identification of Species Involved in Human Infections
Author(s) -
Luís J. del Valle,
Michael L. Jaramillo,
Miguel Talledo,
María J. Pons,
Lidia Flores,
Ruth L. Quispe,
Pablo Ramírez,
Ruth García-de-la-Guarda,
Débora Alvarado,
Abraham Espinoza-Culupú,
Juana del Valle-Mendoza,
Martha Vargas,
Joaquı́m Ruiz
Publication year - 2014
Publication title -
universal journal of microbiology research
Language(s) - English
Resource type - Journals
eISSN - 2331-6721
pISSN - 2331-673X
DOI - 10.13189/ujmr.2014.020102
Subject(s) - identification (biology) , bartonella , 16s ribosomal rna , biology , restriction fragment length polymorphism , microbiology and biotechnology , computational biology , polymerase chain reaction , genetics , bacteria , ecology , gene
We designed a 16S rRNA gene PCR-RFLP scheme to identify all currently described Bartonella spp. The 16S rRNA genes of all Bartonella spp. were in-silico analyzed in order to design a RFLP technique able to discriminate among different species. The restriction enzymes selected were MaeIII, MseI, Sau96I, BsaAI, DrdI, FokI, BssHII, BstUI, AluI, TspDTI and HphI which, according to a decision-making tree, facilitated the differentiation of all the currently described species of Bartonella.The technique was experimentally tested in different species of Bartonella, including human pathogenic B. bacilliformis and B. henselae with a 100% of concordance with the in-silico predicted patterns.This novel RFLP assay could be used to identify both human and non-human pathogenic Bartonella in diagnostic, phylogenetic and epidemiologic studies.
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