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Purpose: Critical thinking is needed in 21st-century learning. The fact is that critical thinking skills are still low. Therefore, the purpose of this study is to investigate critical thinking skills using video learning based on local wisdom Jambi in microbiology courses with regular print modules. Method: Using a quantitative design quasi-experimental post-test control only. Where the total number of samples from this study were 72 biology pre-service teacher education students taken by purposive sampling technique. The experimental group (n = 36) was taught with local wisdom Jambi based learning videos in applied microbiology courses, while the control group (n = 36) was taught using traditional teaching materials. The Oliver-Hoyo Rubric for Critical Thinking (OHRCT) and the attitude questionnaire regarding cultural values are given. The data is then analyzed with the help of the SPSS 21 application to look for descriptive statistics in the form of mean, and inferential categories and statistics for the independent sample t-test. Finding: There is a significant difference in terms of critical thinking and student cap between the control class and the experimental class. It was found that by using video learning based on local wisdom Jambi obtained differences with the t-test value of 15.540 on critical thinking skills. It can be underlined that video learning based on local wisdom Jambi has a significant effect on students' critical thinking skills compared to conventional groups.

High Order Thinking Skills for Biology Education: Applied Microbiology Learning Videos Based on Jambi Local Wisdom