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Vigna radiata (L.) Wilczek is an important legume crop in India for Food as well as Fodder Purpose, enriched with good Proteins, Carbohydrates, Vitamins and Minerals. The demand of Vigna radiata is high in market today, so there is a need to enhance the productivity and area under cultivation. The Mutation Plant Breeding is the most popular, widely used and globally accepted method to improve the cultivars for high yield, early maturity, drought, salinity, heat, insect, disease tolerant/resistant, high nutritional and low antinutritional factors. Mutagens like; Ethyl Methane Sulphonate, Sodium Azide, Gamma Irradiation are used in Mutation Breeding to create variability. The present study has been carried out on the Vigna radiata, Cultivar-Naval. The first stage in the research was the determination of LD50 Value of the Cultivar Naval. It was carried out with different concentrations and time of intervals with mutagens like; EMS, SA and GR with including control as nil (without treatment). The parameters were studied in accordance with the International Seed Testing Association (ISTA) rules. The results showed variation in the studied parameters as compared to control.

Determination of LD<sub>50</sub> Value of Vigna radiata (L.) Wilczek Cultivar- Naval