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1. Why was an elution performed? 2. Which laboratory test should be performed next? 3. Did the transfusion of the second unit cause the patient’s symptoms? 4. Did a transfusion reaction occur and, if so, what kind of reaction was it? 5. How should the clinician be advised regarding the cause of the patient’s symptoms? 6. What further actions are necessary on the part of the laboratory? 7. Why was this not detected prior to the transfusion of blood products? 8. How common is this occurrence?

Evaluation of a Possible Transfusion Reaction With a Positive Direct Antiglobulin Test in a 29-Year-Old Male