Human achaete-scute Homologue (hASH1) mRNA Level as a Diagnostic Marker to Distinguish Esthesioneuroblastoma From Poorly Differentiated Tumors Arising in the Sinonasal Tract | Zendy

Paulette Mhawech | Zendy; Margaret Berczy | Zendy; Mounia Assaly | Zendy; François R. Herrmann | Zendy; Hanifa Bouzourène | Zendy; Abdelkarim S. Allal | Zendy; Pavel Dulguerov | Zendy; Jürg Schwaller | Zendy

Open Access

Human achaete-scute Homologue (hASH1) mRNA Level as a Diagnostic Marker to Distinguish Esthesioneuroblastoma From Poorly Differentiated Tumors Arising in the Sinonasal Tract

Author(s) -

Paulette Mhawech,

Margaret Berczy,

Mounia Assaly,

François R. Herrmann,

Hanifa Bouzourène,

Abdelkarim S. Allal,

Pavel Dulguerov,

Jürg Schwaller

Publication year - 2004

Publication title -

american journal of clinical pathology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.859

H-Index - 128

eISSN - 1943-7722

pISSN - 0002-9173

DOI - 10.1309/qd0k-9q1j-bh6b-5gqq

Subject(s) - esthesioneuroblastoma , biology , messenger rna , nasal cavity , pathology , gene , medicine , genetics , anatomy

Distinction of high-grade esthesioneuroblastomas from other poorly differentiated tumors arising in the nasal cavity is an important diagnostic challenge because it determines patient management and prognosis. The human achaete-scute homologue (hASH1) gene is critical in olfactory neuronal differentiation and is expressed in immature olfactory cells; therefore, it could have potential use as a diagnostic marker The aim of the present study was to determine the value of hASH1 messenger RNA (mRNA) levels in differentiating esthesioneuroblastoma from other poorly differentiated tumors. A real-time polymerase chain reaction assay was developed, permitting the comparative determination of hASH1 mRNA levels in triplicate in a double-blind pilot study including 24 frozen cases of esthesioneuroblastoma and poorly differentiated tumors. All 4 positive cases were esthesioneuroblastomas, and all 19 poorly differentiated tumors were negative. In addition, there was an inverse association between the grade of esthesioneuroblastomas and hASH1 mRNA levels. The hASH1 mRNA level might represent a useful tool for distinguishing esthesioneuroblastoma from poorly differentiated tumors of the sinonasal region.

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