Validation of a Manual Headspace Gas Chromatography Method for Determining Volatile Compounds in Biological Fluids | Zendy

Guilherme Vannucchi Portari | Zendy; Júlio Sérgio Marchini | Zendy; Alceu Afonso Jordão | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Validation of a Manual Headspace Gas Chromatography Method for Determining Volatile Compounds in Biological Fluids

Author(s) -

Guilherme Vannucchi Portari,

Júlio Sérgio Marchini,

Alceu Afonso Jordão

Publication year - 2007

Publication title -

laboratory medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.332

H-Index - 28

eISSN - 1943-7730

pISSN - 0007-5027

DOI - 10.1309/ea876c1pvc0uxph0

Subject(s) - acetaldehyde , chromatography , gas chromatography , acetone , biological fluids , chemistry , methanol , acetonitrile , ethanol , organic chemistry

Background: We report the validation of a method for the determination of acetaldehyde, acetone, methanol, and ethanol in biological fluids using manual headspace sample introduction and an acetonitrile internal standard. Method: This method uses a capillary column (I = 30 m, I.D. = 0.25 mm, dF = 0.25 mu m) installed in a gas chromatography-flame ionization detector (GC-FID) apparatus with a run time of 7.5 minutes. Results: Analysis of the retention times and the resolution of the analyte peaks demonstrated excellent separation without widening of the peaks. Precision and accuracy were good (interassay precision < 15% and recovery between 85% and 115%) in both blood and urine. Conclusion: The method was linear (r > 0.09) over the analytical measurement range (AMR) of each analyte

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research