Diagnostic Role of Circulating Free Plasma DNA Detection in Patients With Localized Prostate Cancer | Zendy

Annalisa Altimari | Zendy; Antonia D’Errico Grigioni | Zendy; Elisa Benedettini | Zendy; Elena Gabusi | Zendy; Riccardo Schiavina | Zendy; Antonio Martinell | Zendy; Antonio Maria MorselliLabate | Zendy; Giuseppe Martorana | Zendy; Walter Franco Grigioni | Zendy; Michelangelo Fiorentino | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Diagnostic Role of Circulating Free Plasma DNA Detection in Patients With Localized Prostate Cancer

Author(s) -

Annalisa Altimari,

Antonia D’Errico Grigioni,

Elisa Benedettini,

Elena Gabusi,

Riccardo Schiavina,

Antonio Martinell,

Antonio Maria MorselliLabate,

Giuseppe Martorana,

Walter Franco Grigioni,

Michelangelo Fiorentino

Publication year - 2008

Publication title -

american journal of clinical pathology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.859

H-Index - 128

eISSN - 1943-7722

pISSN - 0002-9173

DOI - 10.1309/dbpx1mfnddjbw1fl

Subject(s) - prostate cancer , biomarker , medicine , cancer , gstp1 , receiver operating characteristic , stage (stratigraphy) , pathology , prostate , polymerase chain reaction , dna methylation , oncology , gastroenterology , gene , biology , genotype , gene expression , paleontology , biochemistry

To analyze the potential diagnostic relevance of free plasma DNA (FPDNA), we enrolled 64 patients with localized prostate cancer (CaP). FPDNA was quantified by real-time polymerase chain reaction assessment of the HTERT gene in blood samples from 64 patients with CaP and 45 healthy males. Methylation of the GSTP1 gene was used to confirm the neoplastic origin of FPDNA in selected cases. The mean +/- SD levels of FPDNA were higher in patients with CaP (15.4 +/- 10.9 ng/mL) than in control subjects (5.5 +/- 3.5 ng/mL; P <.001). By using the best cutoff value, the sensitivity of the test was 80%, the specificity was 82%, the area under the receiver operating characteristic curve, 0.881. High FPDNA values were significantly associated with pathologic T3 stage (P = . 035). Methylation of the GSTP1 gene was found in 4 (25%) of 16 FPDNA samples and 15 (94%) of 16 tissue samples. Quantification of FPDNA discriminates between patients with CaP and healthy subjects and correlates with pathologic tumor stage. FPDNA is a candidate biomarker for early diagnosis and monitoring of CaP.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research