The effect of varied NAA concentration agains growth and flavonoid content of Gynura procumbens (Lour) Merr. callus

The aim of this research is to study the influence of naphthalene acetic acid (NAA) concentration variation in the callus growth and flavonoid content of daun dewa [Gynura procumbens (Lour) Merr.]. The research framework relied on the benefit of daun dewa as anti cancer drug. This matter enabled by its compound cytotoxic from compound flavonoid contained. Culture in vitro technique serves the purpose of medium to produce the secondary metabolism. Addition of NAA as treatment for the induction of callus will promote cell enlargement and the growth of callus so that callus which later will be analysed by its flavonoid content. This research was conducted with two phase. First phase represented the phase of antecedent attempt in the form of callus induction to get the good callus. Addition of NAA and kinetin conducted to promote the cell proliferation. The parameter of this phase is texture, colour, and moment of callus appearance. Second phase is the form of NAA concentration variation treatment phase. At this research, it conducted with the random device complete with one factor that is 5 concentration NAA is 0 mg/L; 0,5 mg/L; 1,0 mg/L; 1,5 mg/L and 2,0 mg/L. The parameter of this phase is wet weight of the callus, dry weight of the callus, texture of the callus, colour of the callus, and flavonoid content. The result of this research indicated that the addition NAA does not influence on callus growth and the flavonoid content of callus daun dewa.