Open AccessPhytochemical and toxicity evaluation of Phaleria macrocarpa (Scheff.) Boerl by MCF-7 cell line and brine shrimp lethality bioassay
Author(s) -
Abul Kalam Azad
Publication year - 2016
Publication title -
journal of coastal life medicine
Language(s) - English
Resource type - Journals
eISSN - 2309-6152
pISSN - 2309-5288
DOI - 10.12980/jclm.4.2016j5-194
Subject(s) - brine shrimp , phytochemical , bioassay , traditional medicine , saponin , mtt assay , cytotoxicity , mcf 7 , glycoside , viability assay , biology , chemistry , food science , pharmacology , in vitro , botany , biochemistry , medicine , cancer cell , cancer , genetics , alternative medicine , pathology , human breast
Objective: To evaluate the cytotoxicity of Phaleria macrocarpa fruits extracts. Methods: The cytotoxicity test was carried out by in vitro MCF-7 cell line and in vivo brine shrimp lethality bioassay. Results: The preliminary phytochemical test showed the presence of alkaloids, carbohydrate, glycosides, saponin, terpene, steroids, phenols and flavonoids. The MTT-assay results showed that the highest percentage of cell viability was 106.23% at concentration of 1.25 µL and the lowest percentage was 13.04% at concentration of 10 µL. Conclusions: The MTT-assay and brine shrimp lethality bioassay results showed that the extract was non-toxic and it would be consumable as a herbal remedy.
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