Application of UV-Spectrophotometry and RP-HPLC for Simultaneous Determination of Atorvastatin Calcium and Ezetimibe in Pharmaceutical Dosage Form | Zendy

Sandeep Sonawane | Zendy; Atul A. Shirkhedkar | Zendy; R. A. Fursule | Zendy; Sanjay J. Surana | Zendy

Open Access

Application of UV-Spectrophotometry and RP-HPLC for Simultaneous Determination of Atorvastatin Calcium and Ezetimibe in Pharmaceutical Dosage Form

Author(s) -

Sandeep Sonawane,

Atul A. Shirkhedkar,

R. A. Fursule,

Sanjay J. Surana

Publication year - 2006

Publication title -

eurasian journal of analytical chemistry

Language(s) - English

Resource type - Journals

ISSN - 1306-3057

DOI - 10.12973/ejac/77004

Subject(s) - ezetimibe , chromatography , dosage form , chemistry , spectrophotometry , atorvastatin , high performance liquid chromatography , pharmacology , medicine , cholesterol , biochemistry

Two methods are described for the simultaneous determination of Atorvastatin calcium and Ezetimibe in binary mixture. The first method was based on UV-spectrophotometric determination of two drugs, using simultaneous equation method. It involves absorbance measurement at 232.5 nm ( l max of Ezetimibe) and 246.0 nm ( l max of Atorvastatin calcium) in methanol; linearity was obtained in the range of 5 – 25 m g .mL -1 for both the drugs. The second method was based on HPLC separation of the two drugs in reverse phase mode using Luna C 18 column. Linearity was obtained in the concentration range of 8-22 m g .mL -1 for both the drugs. Both these methods have been successively applied to pharmaceutical formulation and were validated according to ICH guidelines.

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