Histochemical Analysis of Expression Pattern of Camv 35S Promoter in Transgenic Rapeseed (Brassica Napus L.) | Zendy

Parissa Jonoubi | Zendy; Ali Hatef Salmanian | Zendy; Amin Ravaei | Zendy

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Histochemical Analysis of Expression Pattern of Camv 35S Promoter in Transgenic Rapeseed (Brassica Napus L.)

Author(s) -

Parissa Jonoubi,

Ali Hatef Salmanian,

Amin Ravaei

Publication year - 2015

Publication title -

current world environment

Language(s) - English

Resource type - Journals

eISSN - 2320-8031

pISSN - 0973-4929

DOI - 10.12944/cwe.10.special-issue1.90

Subject(s) - rapeseed , brassica , transgene , genetically modified crops , biology , microbiology and biotechnology , botany , biochemistry , gene

Promoters are considered valuable tools for biotechnology and provide great opportunities for eugenic purposes. This study aimed to investigate the expression pattern of GUS gene directed by CaMV 35S promoter in transgenic rapeseed (Brassica napus L.). The GUS gene was transferred to the rapeseed explants by Agrobacterium. The regenerated and rooted transgenic plantlets were transferred to the pots containing a mixture of soli and vermiculite. These plants underwent PCR and histochemical GUS assay. The cross-section of leaf, petiole, and stem of the transformed plants was obtained. GUS activity was observed in phloem, parenchyma, collenchyma, and supporting tissue of vascular bundles. It was also observed in cambium, endoderm, vascular ray, pith parenchyma, epidermis, and trichomes. These results showed that CaMV 35S causes the expression of transgene in various tissues differently.

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