Study of Nano-Graphene Oxide Effects on the Number of Kupffer Cells and Megakaryocytes in Liver of NMRI Strain Mouse Embryo in Vivo

To investigate the effects of nano-graphene oxide on the number of kupffer cells and megakaryocytes, in vivo method was applied. In this study, four groups of livers including control, sham, experimental group 1 (using a dose of 17 mg/kg), experimental group 2 (using a dose of 5.5 mg/kg), were investigated. On day 9 of gestation, control group without the effect of graphene oxide, sham group with injection of water as graphene oxide solvent and experimental groups with injection of graphene oxide (1.2 nm particles) with doses of 17 and 5.5 mg/kg mouse weight were examined. Then, on day 15 of gestation, embryos were removed from the mother‘s body and their livers were amputated. The statistical results obtained by counting the number of kupffer cells and megakaryocytes in experimental groups that received nano graphene oxide, showed significant changes as compared with the sham and control groups. In the dose of 17 mg/kg there was a significant increase (P<0.001) in the number of kupffer cells and significant increase in the dose of 5.5 mg/kg (P<0.05) in the number of megakaryocytes. These findings showed the destructive effect of nano-graphene oxide on the development of liver in the condition of in vivo.