Vol 1, no. 2

A centrifuge generates centrifugal force to separate solids from a liquid. Through a combination of speed (rotations per minute – rpm) and the radius of the centrifuge's rotor, the relative centrifugal force (rcf) can be calculated. Optimal rcf ranges have been determined for urine and blood specimens in order to consistently prepare quality specimens for laboratory testing. Centrifuging blood at higher than recommended rpm speeds can result in hemolized blood specimens or cause specimen tubes to break. Lower speeds may not completely separate solids from fluids. Safety Concerns Centrifuges must be located on level, solid work surfaces. To maximize safety, the immediate area around the centrifuge must be kept clear of other objects. The surface must be smooth to allow the suction cups (feet) of the centrifuge to grip the surface and hold the centrifuge in place during operation. Centrifuges are very dangerous when operated improperly. Read the operator's manual and follow instructions to minimize hazards. Tube Breakage If a specimen tube breaks in the centrifuge, turn off the centrifuge immediately. DO NOT open the lid for about 30 minutes. This will allow the fine aerosol droplets to settle. Unplug the centrifuge from the electrical outlet! After 30 minutes have passed, open the lid and carefully remove the broken specimen container using Vol. 1, No. 2 forceps while wearing puncture resistant utility gloves and a lab coat. Recommend you also wear eye protection and a surgical mask to maximize your protection against infectious aerosols and contaminated materials. Fine granules of glass can be collected by using forceps to swab the interior surfaces with wet gauze pads. After all broken glass and specimen material has been removed, disinfect interior and exterior surfaces with a 10% hypochlorite solution (bleach). Allow all surfaces to dry before using the centrifuge. Document this situation on the centrifuge preventive maintenance log sheet. Regardless of the type or purpose of the centrifuge, the basic operational principles are the same. A good preventive maintenance program will insure optimal performance of your centrifuge. These are key preventive maintenance tasks necessary to keep your centrifuge in good working order: Keep it Clean! Wipe up spills as they happen and clean the surface with a disinfectant. Routine cleaning is also necessary. The exterior should be cleaned daily or weekly and the interior should be cleaned on a monthly basis. This includes the rotor, specimen holders, and rubber cushions. Always unplug the …