Open AccessIntrauterine embryo transfer with canine embryos cryopreserved by the slow freezing and the Cryotop method
Author(s) -
Tatsuya Hori,
Hitoshi Ushijima,
Tatsuo Kimura,
Masanori Kobayashi,
Eiichi Kawakami,
Toshihiko Tsutsui
Publication year - 2016
Publication title -
journal of veterinary medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.51
H-Index - 60
eISSN - 1347-7439
pISSN - 0916-7250
DOI - 10.1292/jvms.16-0037
Subject(s) - cryopreservation , cryoprotectant , embryo , andrology , blastocyst , embryo cryopreservation , dimethyl sulfoxide , embryo transfer , biology , chemistry , embryogenesis , medicine , microbiology and biotechnology , organic chemistry
Canine embryos (8-cell to blastocyst stages) frozen-thawed using the slow-freezing method with glycerol (four recipients) or dimethyl sulfoxide (three recipients) as a cryoprotectant and vitrified-warmed using the Cryotop method (five recipients) were surgically transferred into the unilateral uterine horn of recipient bitches. As a result, the morphology of embryos frozen-thawed using the slow-freezing method was judged to be normal, but no conception occurred in any of the recipient bitches. Two of the five bitches that received transferred embryos (morula to early blastocyst stages) vitrified-warmed using the Cryotop method became pregnant and produced normal pups (1/9 embryos, 11.1% and 1/6 embryos, 17.0%). It was concluded that the Cryotop method was more appropriate for canine embryo cryopreservation than the slow-freezing method, which is used for the cryopreservation of embryos of other mammalian species.