DNA single-strand breaks and cytotoxicity induced by chromate(VI), cadmium(II), and mercury(II) in hydrogen peroxide-resistant cell lines. | Zendy

Katsuyuki Tsuzuki | Zendy; Masayasu Sugiyama | Zendy; Nobuya Haramaki | Zendy

Open Access

DNA single-strand breaks and cytotoxicity induced by chromate(VI), cadmium(II), and mercury(II) in hydrogen peroxide-resistant cell lines.

Author(s) -

Katsuyuki Tsuzuki,

Masayasu Sugiyama,

Nobuya Haramaki

Publication year - 1994

Publication title -

environmental health perspectives

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.257

H-Index - 282

eISSN - 1552-9924

pISSN - 0091-6765

DOI - 10.1289/ehp.94102s3341

Subject(s) - chinese hamster ovary cell , cytotoxicity , cadmium , microbiology and biotechnology , chromium , hydrogen peroxide , dna , dna damage , chemistry , cadmium chloride , chromate conversion coating , cell culture , biology , biochemistry , in vitro , genetics , organic chemistry

The induction of cytotoxicity and DNA single-strand breaks by chromium(VI), cadmium(II), and mercury(II) were compared in H2O2-resistant Chinese hamster ovary (CHO(R)) cells and parental (CHO(P)) cells. Using a colony-forming assay, CHO(R) cells were found to be significantly more resistant than CHO(P) cells to the cytotoxicity caused by CdCl2 and HgCl2, but not to that caused by Na2CrO4. However, the DNA single-strand breaks produced by each of these metals were significantly lower in the CHO(R) cells. With respect to chromium reduction, the level of chromium(V) in CHO(R) cells was decreased. The role of intracellular active oxygen in the heavy metal-induced DNA damage and cytotoxicity is discussed.

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