Interference of cadmium with ATP-stimulated nuclear calcium uptake. | Zendy

S. Hechtenberg | Zendy; Detmar Beyersmann | Zendy

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Interference of cadmium with ATP-stimulated nuclear calcium uptake.

Author(s) -

S. Hechtenberg,

Detmar Beyersmann

Publication year - 1994

Publication title -

environmental health perspectives

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.257

H-Index - 282

eISSN - 1552-9924

pISSN - 0091-6765

DOI - 10.1289/ehp.94102s3265

Subject(s) - thapsigargin , intracellular , microbiology and biotechnology , signal transduction , nuclear transport , calcium in biology , atpase , calcium , biology , cell growth , chemistry , biophysics , biochemistry , cell nucleus , enzyme , cytoplasm , organic chemistry

The spatial and temporal regulation of intracellular free Ca2+ serves as a modulator of signal transduction pathways involved in cell growth and differentiation. Thus, interference of metals with intracellular Ca2+ homeostasis has been considered as a target of toxic action. We used the fluorescence indicator fura-2 to monitor the level of free Ca2+ in isolated bovine liver nuclei. Nuclei accumulated Ca2+ by an ATP-stimulated Ca2+ uptake system, which is sensitive to inhibition by thapsigargin, a specific inhibitor of intracellular Ca(2+)-ATPases. Preincubation of nuclei with nanomolar concentrations of free Cd2+ resulted in a dose-dependent inhibition of ATP-dependent nuclear Ca2+ uptake. We conclude that impairment of nuclear Ca2+ regulation caused by Cd2+ provokes alterations in nuclear events related to gene expression and cell proliferation.

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