Aqueous extracts of cigarette tar containing the tar free radical cause DNA nicks in mammalian cells. | Zendy

K K Stone | Zendy; Elena Bermúdez | Zendy; W. A. PRYOR | Zendy

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Aqueous extracts of cigarette tar containing the tar free radical cause DNA nicks in mammalian cells.

Author(s) -

K K Stone,

Elena Bermúdez,

W. A. PRYOR

Publication year - 1994

Publication title -

environmental health perspectives

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.257

H-Index - 282

eISSN - 1552-9924

pISSN - 0091-6765

DOI - 10.1289/ehp.94102s10173

Subject(s) - tar (computing) , chemistry , dna , aqueous solution , dna damage , biochemistry , semiquinone , biophysics , microbiology and biotechnology , organic chemistry , biology , computer science , programming language , quinone

The ability of aqueous extracts of cigarette tar to nick DNA was investigated using viable mammalian cells. Tar extracts contain a radical with a stable electron spin resonance (ESR) signal at g = 2.0036 characteristic of a semiquinone. The association of the tar component that carries the ESR signal with DNA was demonstrated using viable rat alveolar macrophages. The formation of single-strand DNA breaks caused by cigarette tar extracts in viable rat thymocytes follows saturation kinetics, indicating a tar component associates with DNA and then nicks it. These studies support our hypothesis that tar components that contain the cigarette tar radical can enter cells, associate with, and then nick DNA.

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