Open AccessSynthetic tumor-associated glycopeptide antigens.
Author(s) -
Horst Kunz,
Peter Wernig,
Marita Schilling,
Joachim März,
Carlo Unverzagt,
Stefan Birnbach,
Uwe Lang,
Herbert Waldmann
Publication year - 1990
Publication title -
environmental health perspectives
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 2.257
H-Index - 282
eISSN - 1552-9924
pISSN - 0091-6765
DOI - 10.1289/ehp.9088247
Subject(s) - glycopeptide , antigen , bovine serum albumin , chemistry , biochemistry , anomer , glycoprotein , peptide , serum albumin , stereochemistry , biology , immunology , antibiotics
Glycopeptides with TN antigen (GalNAc)Ser/Thr and T-antigen structures (beta Gall-3GalNAc)Ser/Thr, described as tumor-associated antigens, were synthesized and coupled to bovine serum albumin. Alternatively, synthetic methods for the construction of beta-anomeric analogues of the TN and T-antigen glycopeptides were developed, aiming at antigenic structures having a varied stereochemistry of the linkage between the carbohydrate and the peptide moiety. As a further type of potential tumor-associated antigen, fucosyl-chitobiose asparagine glycopeptides were synthesized, deprotected, and coupled to bovine serum albumin. The chemical methods developed now make the complex sensitive glycoprotein partial structures accessible in analytically pure form and in preparative amounts.