Development of HPLC methods for the purification and analysis of plasma membrane glycoproteins. | Zendy

Doris Renauer | Zendy; H.U. Gierlich | Zendy; Klaus K. Unger | Zendy

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Development of HPLC methods for the purification and analysis of plasma membrane glycoproteins.

Author(s) -

Doris Renauer,

H.U. Gierlich,

Klaus K. Unger

Publication year - 1990

Publication title -

environmental health perspectives

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.257

H-Index - 282

eISSN - 1552-9924

pISSN - 0091-6765

DOI - 10.1289/ehp.9088243

Subject(s) - chromatography , glycoprotein , chemistry , affinity chromatography , high performance liquid chromatography , membrane , ion chromatography , resolution (logic) , size exclusion chromatography , membrane glycoproteins , hydrophilic interaction chromatography , biochemistry , enzyme , artificial intelligence , computer science

High resolution HPLC techniques such as affinity chromatography (AC), ion exchange chromatography (IEC), and size exclusion chromatography (SEC) were used successfully for separations of hydrophobic plasma membrane glycoproteins. We have tested a lot of commercially available columns for IEC and SEC and performed the purification of the crude plasma membrane extract with the most suitable columns. By using immobilized ligands with different specificities and sequential affinity chromatography, it is possible to obtain a preliminary structural characterization of the interesting carbohydrate residues of membrane glycoproteins.

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