Measurement of cytosolic calcium using 19F NMR. | Zendy

Elizabeth Murphy | Zendy; L A Levy | Zendy; B. Raju | Zendy; Charles Steenbergen | Zendy; J. T. Gerig | Zendy; P. Singh | Zendy; Robert E. London | Zendy

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Measurement of cytosolic calcium using 19F NMR.

Author(s) -

Elizabeth Murphy,

L A Levy,

B. Raju,

Charles Steenbergen,

J. T. Gerig,

P. Singh,

Robert E. London

Publication year - 1990

Publication title -

environmental health perspectives

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.257

H-Index - 282

eISSN - 1552-9924

pISSN - 0091-6765

DOI - 10.1289/ehp.908495

Subject(s) - cytosol , calcium , ischemia , chemistry , cell , biophysics , nuclear magnetic resonance , biochemistry , medicine , biology , organic chemistry , enzyme , physics

Fluorine-19 nuclear magnetic resonance (NMR) studies of cells and perfused organs loaded with fluorinated ion chelators represent a new approach to determining cytosolic free calcium levels in situ. The molecular basis for this approach and the relative advantages and disadvantages of the NMR technique are discussed in this paper. Results obtained on perfused normoxic and ischemic rat hearts are presented, indicating that ischemia is associated with an elevation in the level of cytosolic free calcium before the onset of irreversible cell injury. The results are therefore consistent with this elevation playing a causative role in the mediation of myocardial cell injury resulting from ischemia.

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