Chemical modification of DNA with muta-carcinogens. III. Reductive alkylation of DNA with mitomycin C. | Zendy

Y Hashimoto | Zendy; Koichi Shudo | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Chemical modification of DNA with muta-carcinogens. III. Reductive alkylation of DNA with mitomycin C.

Author(s) -

Y Hashimoto,

Koichi Shudo

Publication year - 1985

Publication title -

environmental health perspectives

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.257

H-Index - 282

eISSN - 1552-9924

pISSN - 0091-6765

DOI - 10.1289/ehp.8562219

Subject(s) - nucleotide , dna , alkylation , chemistry , mitomycin c , hydrolysis , carcinogen , enzyme , stereochemistry , biochemistry , catalysis , biology , genetics , gene

Mitomycin C (MMC) binds to DNA after its reductive activation by catalytic hydrogenation with Pd on charcoal. Three modified nucleotides, named MG-1, MG-2, and MA, were isolated from the modified DNA after enzymatic hydrolysis to 5'-nucleotides. The structures of these modified nucleotides were deduced from their 1H-NMR and UV spectra, and from studies of the chemically transformed derivatives (hydrolysis, methylation, diazotization, and thioketonization). These three modified nucleotides were concluded to be 1,2-trans-2,7-diamino-1-(N2-deoxyguanylyl)mitosene (MG-1), 2,7-diamino-1-(O6-deoxyguanylyl)mitosene (MG-2) and 2,7-diamino-1-(N6-deoxyadenylyl)mitosene (MA). The same modified nucleotides were identified in DNA extracted from the livers of rats treated with MMC.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research