Adh locus in maize for detection of mutagens in the environment. | Zendy

Drew Schwartz | Zendy

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Adh locus in maize for detection of mutagens in the environment.

Author(s) -

Drew Schwartz

Publication year - 1981

Publication title -

environmental health perspectives

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.257

H-Index - 282

eISSN - 1552-9924

pISSN - 0091-6765

DOI - 10.1289/ehp.813775

Subject(s) - biology , locus (genetics) , mutagenesis , mutant , genetics , enzyme , phytic acid , biochemistry , chemistry , gene

The advantages of the maize Adh system for low level mutagenesis studies are discussed. They include high mutation rate and the availability of both a strong selection system for null mutants and a sensitive method for detection of variants. The null selection involves treatment of pollen with allyl alcohol which is converted by the Adh enzyme to the toxic acrylaldehyde. Adh variants can be detected by alteration in affinity for phytic acid as well as thermal stability and electrophoretic mobility.

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