Isolation of cells that retain differentiated functions in vitro: properties of clonally isolated type II alveolar pneymonocytes. | Zendy

W.H. Douglas | Zendy; Philip M. Farrell | Zendy

Open Access

Isolation of cells that retain differentiated functions in vitro: properties of clonally isolated type II alveolar pneymonocytes.

Author(s) -

W.H. Douglas,

Philip M. Farrell

Publication year - 1976

Publication title -

environmental health perspectives

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.257

H-Index - 282

eISSN - 1552-9924

pISSN - 0091-6765

DOI - 10.1289/ehp.761683

Subject(s) - lamellar granule , in vitro , alveolar cells , lung , ploidy , cytoplasm , microbiology and biotechnology , biology , cell culture , cell type , cellular differentiation , isolation (microbiology) , a549 cell , cell , chemistry , biochemistry , pulmonary surfactant , genetics , medicine , gene

We have isolated byclonal culture techniques a diploid cell strain (L-2) from adult rat lung. These cells appear to retain differentiated functions that are present in type II alveolar epithelial cells of intact lung. The L-2 cells are diploid, epithelial cells, they contain osmiophilic lamellar bodies in their cytoplasm and they synthesize lecithin by the same de novo pathways as whole lung.

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