Open AccessResponse of cell cultures to asbestos fibers
Author(s) -
Joachim Bruch
Publication year - 1974
Publication title -
environmental health perspectives
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.257
H-Index - 282
eISSN - 1552-9924
pISSN - 0091-6765
DOI - 10.1289/ehp.749253
Subject(s) - asbestos , erythrosine , chemistry , staining , biophysics , cytotoxic t cell , electron microscope , asbestos fibers , pathology , biology , materials science , medicine , biochemistry , in vitro , chromatography , composite material , physics , optics
The reaction of macrophages to asbestos and glass fibers was studied by light and by electron microscopy. Measurements were made of LDH release, staining with erythrosine B, and lactate production. Size and shape of asbestos fibers were found to have more effect than the physicochemical properties of the surface. Long fibers resulted in a protracted pathogenic but no acute cytotoxic effect.