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An in vitro steroidogenesis assay using the human adrenocortical carcinoma cell line H295R is being evaluated as a possible screening assay to detect and assess the impact of endocrine-active chemicals (EACs) capable of altering steroid biosynthesis. Data interpretation and their quantitative use in human and ecological risk assessments can be enhanced with mechanistic computational models to help define mechanisms of action and improve understanding of intracellular concentration-response behavior.

environmental health perspectives

Computational Model of Steroidogenesis in Human H295R Cells toPredict Biochemical Response to Endocrine-Active Chemicals: Model Developmentfor Metyrapone