Heat Shock Protein 70 extraction from human tooth for ELISA

Protein extraction from bone is extremely difficult task because the bone tissue is hard to dissolve. Special care must be taken during extraction to prevent protein degradation and/or unfolding. Boiling is a traditional method for extracting protein from bones, but heat denaturation during this process destroys tertiary structure of some proteins irreversibly.  Dental sciences employ tooth based research and extraction of proteins or other macromolecules from tooth are required most of the time. A new method was developed in our laboratory to extract proteins from tooth. This method overcomes heat or chemical denaturation of proteins and requires couple days. Although the method extract relatively less protein, the total amount of protein is sufficient for ELISA and proteomics. Keywords: Heat Shock Protein 70, tooth, ELISA Ozet ELISA icin insan disinden isi sok protein 70 ekstraksyonu Kemikten protein ekstraksyonu kemik dokusunun cozulmesi oldukca kisitli oldugundan zor bir islemdir. Ekstraksyon sirasinda protein yikimi ve/veya katlanmasini engellemek icin ozel onlem alinmalidir. Kaynatmak kemiklerden protein cikartmak icin geleneksel bir yontemdir fakat bu islem suresince isi kaynakli denaturasyon bazi proteinleri geri donusumsuz ucuncul yapilarini bozarlar. Dis bilimleri cogu kez dis ile ilgili calismalar,  disten protein veya diger molekullerin ekstraksiyonunu gerektirir. Laboratuarimizda disten protein ekstraksyonu icin yeni bir metot gelistirildi.  Bu metot isi ve kimyasal denaturasyonun onune gecer ve bir kac gun gerektirir. Yeni metot goreceli az protein ekstrakte etsede, elde edilen toplam protein ELISA ve proteomik calismalari icin yeterlidir. Anahtar Sozcukler: Isi sok protein 70, dis, ELISA