Protective Role of Alginic Acid and Fucoidan from Nitric Oxide-Induced Oxidative Stress

The protective activities of alginic acid and fucoidan from oxidative stress (OS) were investigated under cellular system using LLC-PK1 renal epithelial cells that are widely used for study cellular OS. The cellular OS in LLC-PK1 cells was induced by the free radical generators. The treatment of pyrogallol, sodium nitroprusside or 3-morpholinosydnonimine led to the production of superoxide anion (O2-), nitric oxide (NO) and peroxynitrite (ONOO-), respectively. The free radical generators decreased cell viability and elevated lipid peroxidation compared OSwith non-treated cells. However, the treatment of alginic acid and fucoidan attenuated OS significantly induced by NO, O2- and ONOO-, resulting in the significant increase of cell viability and inhibition of lipid peroxidation in concentration-dependent manner. In particular, fucoidan showed the stronger protective effect from oxidative stress than alginic acid. These results indicated that alginic acid and fucoidan played the protective role from NO-induced OS. The present study suggests the promising antioxidative agents of alginc acid and fucoidan with protective activity from free radical-induced OS.