The Unique Kinetic Behavior of the Very Large NAD-Dependent Glutamate Dehydrogenase fromJanthinobacterium lividum | Zendy

Ryushi Kawakami | Zendy; Masaki Oyama | Zendy; Haruhiko Sakuraba | Zendy; Toshihisa Ohshima | Zendy

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The Unique Kinetic Behavior of the Very Large NAD-Dependent Glutamate Dehydrogenase fromJanthinobacterium lividum

Author(s) -

Ryushi Kawakami,

Masaki Oyama,

Haruhiko Sakuraba,

Toshihisa Ohshima

Publication year - 2010

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.90925

Subject(s) - nad+ kinase , glutamate dehydrogenase , kinetic energy , chemistry , biology , physics , glutamate receptor , biochemistry , enzyme , classical mechanics , receptor

The kinetics of a very large NAD-dependent glutamate dehydrogenase from Janthinobacterium lividum showed positive cooperativity toward alpha-ketoglutarate and NADH, and the Michaelis-Menten type toward ammonium chloride in the absence of the catalytic activator, L-aspartate. An increase in the maximum activity accompanied the decrease in the S(0.5) values for alpha-ketoglutarate and NADH with the addition of L-aspartate, and the kinetic response for alpha-ketoglutarate changed completely to a typical Michaelis-Menten type in the presence of 10 mM L-aspartate.

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