Immunochemical Detection of Food-Derived Isothiocyanate as a Lysine Conjugate | Zendy

Toshiyuki Nakamura | Zendy; Noritoshi Kitamoto | Zendy; Toshihiko Osawa | Zendy; Yoji Kato | Zendy

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Immunochemical Detection of Food-Derived Isothiocyanate as a Lysine Conjugate

Author(s) -

Toshiyuki Nakamura,

Noritoshi Kitamoto,

Toshihiko Osawa,

Yoji Kato

Publication year - 2010

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.90728

Subject(s) - monoclonal antibody , conjugate , chemistry , allyl isothiocyanate , isothiocyanate , benzyl isothiocyanate , lysine , antibody , bovine serum albumin , carica , in vitro , fluorescein isothiocyanate , papaya ringspot virus , biochemistry , bioavailability , chromatography , pharmacology , biology , fluorescence , botany , immunology , amino acid , physics , mathematics , quantum mechanics , coat protein , rna , gene , mathematical analysis

In a previous study we prepared monoclonal antibody against allyl isothiocyanate (AITC)-modified lysine (Lys), and found that AITC reacted with Lys under physiological conditions in vitro (T. Nakamura et al., Chem. Res. Toxicol., 22, 536-542 (2009)). In the present study, antibodies against benzyl isothiocyanate (ITC), 6-methylsulfinylhexyl ITC and phenethyl ITC modified protein were prepared, and the respective monoclonal antibodies, B6C9, 6MS3D10, and PE3A10 were obtained. These antibodies were applied to ITC detection in food using shredded Wasabia japonica (wasabi) and ground Carica papaya (papaya) seed by trapping ITC with biotin-labeled bovine serum albumin. ITC formation from the wasabi and papaya seed samples was confirmed using the antibodies in a dose-dependent manner. These antibodies might be applicable in identifying food-derived ITC.

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