Development of Gateway Binary Vectors, R4L1pGWBs, for Promoter Analysis in Higher Plants | Zendy

Shinya Nakamura | Zendy; Akihide Nakao | Zendy; Makoto Kawamukai | Zendy; Tetsuya Kimura | Zendy; Sumie Ishiguro | Zendy; Tsuyoshi Nakagawa | Zendy

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Development of Gateway Binary Vectors, R4L1pGWBs, for Promoter Analysis in Higher Plants

Author(s) -

Shinya Nakamura,

Akihide Nakao,

Makoto Kawamukai,

Tetsuya Kimura,

Sumie Ishiguro,

Tsuyoshi Nakagawa

Publication year - 2009

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.90720

Subject(s) - cyan , green fluorescent protein , fluorescent protein , yellow fluorescent protein , transformation (genetics) , reporter gene , fluorescence , gateway (web page) , clone (java method) , promoter , biology , microbiology and biotechnology , computational biology , genetics , gene , computer science , physics , gene expression , quantum mechanics , world wide web , optics

We developed a new series of Gateway binary vectors for plant transformation, R4L1pGWBs, which allow easy construction of promoter:reporter clones. R4L1pGWBs contain a recombination attR4-attL1-reporter cassette, and thus an attL4-promoter-attR1 entry clone was efficiently incorporated by the Gateway LR reaction, resulting in the generation of an attB4-promoter-attB1-reporter construct. The reporters employed in R4L1pGWBs were beta-glucuronidase (GUS), luciferase (LUC), enhanced yellow fluorescent protein (EYFP), enhanced cyan fluorescent protein (ECFP), G3 green fluorescent protein (G3GFP), G3GFP-GUS, and tag red fluorescent protein (TagRFP).

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