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Bacillus sp. PS3 produces a glycosylated flagellin. In this study, a number of the glycosylated residues of the flagellin protein were found to be located in the central variable region of this protein. We also report that the motility defect of the Bacillus subtilis flagellin mutant was complemented by Bacillus sp. PS3 flagellin variants without glycosylation, which contained amino acid substitutions and intragenic duplications in the variable region of flagellin.

bioscience, biotechnology, and biochemistry

Amino Acid Substitutions and Intragenic Duplications of<i>Bacillus</i>sp. PS3 Flagellin Cause Complementation of the<i>Bacillus subtilis</i>Flagellin Deletion Mutant

Amino Acid Substitutions and Intragenic Duplications ofBacillussp. PS3 Flagellin Cause Complementation of theBacillus subtilisFlagellin Deletion Mutant