Differential Expression of ThreelabialGenes during Earthworm Head Regeneration
Author(s) -
SungJin Cho,
KiSeok Koh,
Eun Lee,
Soon Cheol Park
Publication year - 2009
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.90416
Subject(s) - earthworm , blastema , regeneration (biology) , biology , reverse transcription polymerase chain reaction , head (geology) , gene , cloning (programming) , transcription (linguistics) , gene expression , anatomy , microbiology and biotechnology , genetics , computer science , paleontology , programming language , linguistics , philosophy
The earthworm provides an excellent model for investigating regeneration. Here we report the full-length cloning of three labial genes (Pex-lab01, Pex-lab02, and Pex-lab03) in the earthworm Perionyx excavatus. To analyze their expression pattern during head and tail regeneration, we used the reverse transcription-polymerase chain reaction. Our results indicate that the three labial genes were expressed only in the head-regenerating tissues. Also, we found that the expression of Pex-lab01 and Pex-lab02 is up-regulated, and this indicates their involvement in wound healing and the blastema formation processes during early head regeneration.
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