Open AccessAntihypertensive Effect of Enzymatic Hydrolysate of Collagen and Gly-Pro in Spontaneously Hypertensive Rats
Author(s) -
Toshiaki Ichimura,
Akiko Yamanaka,
Tatsuro Otsuka,
Eiichi Yamashita,
Susumu Maruyama
Publication year - 2009
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.90197
Subject(s) - hydrolysate , blood pressure , ic50 , oral administration , enzyme , chemistry , angiotensin converting enzyme , renin–angiotensin system , peptide , pharmacology , spontaneously hypertensive rat , inhibitory postsynaptic potential , endocrinology , medicine , enzyme inhibitor , biochemistry , in vitro , hydrolysis
Continuous oral feeding of enzymatic hydrolysate of porcine skin collagen showed an antihypertensive effect in spontaneously hypertensive rats (SHRs). We isolated an angiotensin I-converting enzyme (ACE) inhibitory peptide, Gly-Phe-Hyp-Gly-Pro (IC(50)=91 microM), from the hydrolysate, but the ACE inhibitory activities of the other peptides isolated were weak. Although the ACE inhibitory activity of Gly-Pro (IC(50)=360 microM) was not potent, Gly-Pro exists in collagen as a large number of repeated sequences. We then examined the antihypertensive effect of Gly-Pro. Orally administered Gly-Pro at 500 mg/kg significantly decreased the blood pressure of SHRs, and at 50 mg/kg it also showed a tendency to lower the blood pressure. Oral administration of Gly-Phe-Hyp-Gly-Pro (10 or 30 mg/kg) also decreased the blood pressure of SHRs.
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