Effects of Ca2+on Refolding of the Recombinant Hemolytic Lectin CEL-III | Zendy

Keigo Hisamatsu | Zendy; Hideaki Unno | Zendy; Shuichiro Goda | Zendy; Tomomitsu Hatakeyama | Zendy

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Effects of Ca²⁺on Refolding of the Recombinant Hemolytic Lectin CEL-III

Author(s) -

Keigo Hisamatsu,

Hideaki Unno,

Shuichiro Goda,

Tomomitsu Hatakeyama

Publication year - 2009

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.80793

Subject(s) - recombinant dna , lectin , escherichia coli , chemistry , folding (dsp implementation) , biochemistry , protein folding , microbiology and biotechnology , biology , gene , electrical engineering , engineering

CEL-III is a hemolytic lectin isolated from Cucumaria echinata. Although recombinant CEL-III (rCEL-III) expressed in Escherichia coli showed very weak hemolytic activity compared with native protein, it was considerably enhanced by refolding in the presence of Ca(2+). This suggests that Ca(2+) supported correct folding of the carbohydrate-binding domains of rCEL-III, leading to effective binding to the cell surface and subsequent self-oligomerization.

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