Cloning and Characterization of cDNAs Encodingent-Copalyl Diphosphate Synthases in Wheat: Insight into the Evolution of Rice Phytoalexin Biosynthetic Genes | Zendy

Tomonobu Toyomasu | Zendy; Takuma Kagahara | Zendy; Yuko Hirose | Zendy; Masami Usui | Zendy; Shiho Abe | Zendy; Kazunori Okada | Zendy; Jinichiro Koga | Zendy; Wataru Mitsuhashi | Zendy; Hisakazu Yamane | Zendy

Open Access

Cloning and Characterization of cDNAs Encodingent-Copalyl Diphosphate Synthases in Wheat: Insight into the Evolution of Rice Phytoalexin Biosynthetic Genes

Author(s) -

Tomonobu Toyomasu,

Takuma Kagahara,

Yuko Hirose,

Masami Usui,

Shiho Abe,

Kazunori Okada,

Jinichiro Koga,

Wataru Mitsuhashi,

Hisakazu Yamane

Publication year - 2009

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.80781

Subject(s) - gene , biology , phytoalexin , cloning (programming) , diterpene , biosynthesis , cyclase , molecular cloning , biochemistry , enzyme , genetics , complementary dna , computer science , resveratrol , programming language

In vitro assays using recombinant enzymes enabled three cDNAs encoding ent-copalyl diphosphate synthases to be identified in wheat (Triticum aestivum): TaCPS1, TaCPS2, and TaCPS3. The phylogenetic tree and expression analyses suggest that TaCPS3 is responsible for gibberellin biosynthesis, while TaCPS1 and TaCPS2 are possible functional homologs of diterpene cyclase genes OsCPS2 and OsCPS4 involved in phytoalexin biosynthesis in rice.

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