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Purification and Characterization of an Extracellular Laccase fromPhlebia radiataStrain BP-11-2 That Decolorizes Fungal Melanin
Author(s) -
Shuji Kaneko,
Minyi Cheng,
Hiroshi Murai,
Shinji Takenaka,
Shuichiro Murakami,
Kenji Aoki
Publication year - 2009
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.80740
Subject(s) - laccase , radiata , extracellular , melanin , enzyme , strain (injury) , chemistry , substrate (aquarium) , biochemistry , biology , botany , ecology , anatomy , vigna
Phlebia radiata strain BP-12-2, isolated from forest soil, decolorized fungal melanin and produced an extracellular laccase in culture. The enzyme was purified to homogeneity and characterized. It showed no absorption bands in the ultraviolet above 300 nm or visible regions. It differed from the reported laccases in substrate specificity, kinetic parameters, and NH2-terminal amino acid sequences.

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