Transformation ofArabidopsiswith Plant-Derived DNA Sequences Necessary for Selecting Transformants and Driving an Objective Gene
Author(s) -
Aftab Ahmad,
Izumi Kaji,
Yukiko Murakami,
Nana FUNATO,
Takeshi Ogawa,
Masanori Shimizu,
Yasuo Niwa,
Hirokazu Kobayashi
Publication year - 2009
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.80725
Subject(s) - selectable marker , transformation (genetics) , promoter , gene , genetics , biology , arabidopsis , arabidopsis thaliana , acetolactate synthase , marker gene , dna , computational biology , gene expression , mutant
Most genetically modified plants are made with antibiotic-resistant genes and promoters derived from plant pathogens. To promote public acceptance, we have adapted a selectable marker and promoters all of which were derived from Arabidopsis thaliana to transformation. We have employed the gene for acetolactate synthase with its own promoter as a selectable marker, and the actin 2 gene promoter to strongly express an objective gene.
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