An Antioxidant of Dried Chili Pepper Maintained Its Activity through Postharvest Ripening for 18 Months
Author(s) -
Yusuke OGISO,
Ritsuko HosodaYabe,
Yoshiyuki Kawamoto,
Toshiaki KAWAMOTO,
Koji Kato,
Tomio Yabe
Publication year - 2008
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.80464
Subject(s) - postharvest , ripening , antioxidant , dpph , chemistry , pepper , food science , size exclusion chromatography , high performance liquid chromatography , chromatography , botany , biochemistry , biology , enzyme
The antioxidant properties of hot-water extracts from a dried chili pepper were maintained through the postharvest ripening process at 10 degrees C for 18 months. In order to isolate the antioxidant from the ripe pepper, we fractionated hot-water extracts by size-exclusion gel chromatography. A certain fraction showed antioxidative activity via the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity assay. Structural analysis by reversed-phase high performance liquid chromatography (HPLC), LC-MS, and nuclear magnetic resonance (NMR) revealed that the antioxidant was a known compound, p-coumaryl alcohol. This study indicates that an effective antioxidant in chili pepper sustains its antioxidative effects during the postharvest ripening process.
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