Construction of a Binary Vector for Transformation ofArabidopsis thalianawith a New Selection Marker | Zendy

Nozomu Koizumi | Zendy; Yuji Iwata | Zendy

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Construction of a Binary Vector for Transformation ofArabidopsis thalianawith a New Selection Marker

Author(s) -

Nozomu Koizumi,

Yuji Iwata

Publication year - 2008

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.80428

Subject(s) - transformation (genetics) , arabidopsis thaliana , transgene , green fluorescent protein , plasmid , arabidopsis , gene , biology , selection (genetic algorithm) , tunicamycin , microbiology and biotechnology , expression vector , genetics , recombinant dna , mutant , computer science , unfolded protein response , artificial intelligence

A new binary vector, pZT4B, containing the UDP-N-acetylglucosamine: dolichol phosphate N-acetylglucosamine-1-P transferase (GPT) gene as a selection marker, was constructed. The green fluorescent protein (GFP) gene was inserted into pZT4B, and the resulting plasmid was used in the transformation of Arabidopsis. All of six independent transformants obtained after selection with 0.3 mg/l tunicamycin contained the transgene and showed GFP fluorescence.

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