English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

A novel matrix protein named calcification-associated soluble protein-2 (Casp-2) was isolated from the acetic acid-soluble fraction of the exoskeleton of the crayfish Procambarus clarkii, and its primary structure was determined by a combination of peptide sequencing, mass spectral analysis, and cDNA cloning. Casp-2 consists of 117 amino acid residues and has a chitin-binding consensus sequence, the so-called Rebers-Riddiford (R-R) consensus sequence. Casp-2 exhibited an inhibitory activity on calcium carbonate precipitation from its supersaturated solution in vitro, suggesting association with calcification of the exoskeleton. Reverse transcription PCR and Northern blot analyses indicated that the Casp-2 gene was expressed only at the epidermis throughout the molting stages, and most strongly at the late pre-molt stage. Recombinant Casp-2 showed weak affinity to chitin in spite of having the R-R consensus sequence. These results indicate that Casp-2 interacts loosely with chitin fibrils and regulates calcification in the cuticle.

bioscience, biotechnology, and biochemistry

Structural Determination and Functional Analysis of a Soluble Matrix Protein Associated with Calcification of the Exoskeleton of the Crayfish,<i>Procambarus clarkii</i>

Structural Determination and Functional Analysis of a Soluble Matrix Protein Associated with Calcification of the Exoskeleton of the Crayfish,Procambarus clarkii