Characterization ofBacillus haloduransα-Galactosidase Mel4A Encoded by themel4AGene (BH2228) | Zendy

Andian Ari Anggraeni | Zendy; Makiko Sakka | Zendy; Tetsuya Kimura | Zendy; Khanok RATANAKHAOKCHAI | Zendy; Motomitsu Kitaoka | Zendy; Kazuo Sakka | Zendy

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Characterization ofBacillus haloduransα-Galactosidase Mel4A Encoded by themel4AGene (BH2228)

Author(s) -

Andian Ari Anggraeni,

Makiko Sakka,

Tetsuya Kimura,

Khanok RATANAKHAOKCHAI,

Motomitsu Kitaoka,

Kazuo Sakka

Publication year - 2008

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.80242

Subject(s) - raffinose , divalent , recombinant dna , dithiothreitol , chemistry , escherichia coli , biochemistry , regulon , gene , enzyme , biology , microbiology and biotechnology , organic chemistry , sucrose

A family-4 alpha-galactosidase Mel4A of Bacillus halodurans was expressed in Escherichia coli and characterized. Recombinant enzyme rMel4A depended on NAD+, some divalent cations such as Mn2+, and reducing reagents such as dithiothreitol. rMel4A was active on small saccharides such as raffinose but not on highly polymerized galactomannan. Immunological analysis indicated that raffinose induced the production of Mel4A in B. halodurans.

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