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Disruption of an SCS2 ortholog impaired the growth of the alkane-assimilating yeast Yarrowia lipolytica on n-alkanes, particularly on n-decane, although the mRNA level of the ALK1 gene encoding a highly inducible cytochrome P450ALK was not much affected. The same disruption did not cause inositol auxotrophy, implying that Y. lipolytica SCS2 has a different function from its Saccharomyces cerevisiae counterpart.

bioscience, biotechnology, and biochemistry

Disruption of theSCS2Ortholog in the Alkane-Assimilating YeastYarrowia lipolyticaImpairs Its Growth onn-Decane, but Does Not Impair Inositol Prototrophy