Identification and Characterization ofN-Acylhomoserine Lactone-Acylase from the Fish IntestinalShewanellasp. Strain MIB015 | Zendy

Tomohiro Morohoshi | Zendy; Shigehisa Nakazawa | Zendy; Atsushi Ebata | Zendy; Norihiro Kato | Zendy; Tsukasa Ikeda | Zendy

Open Access

Identification and Characterization ofN-Acylhomoserine Lactone-Acylase from the Fish IntestinalShewanellasp. Strain MIB015

Author(s) -

Tomohiro Morohoshi,

Shigehisa Nakazawa,

Atsushi Ebata,

Norihiro Kato,

Tsukasa Ikeda

Publication year - 2008

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.80139

Subject(s) - quorum sensing , vibrio anguillarum , shewanella , quorum quenching , shewanella oneidensis , biofilm , microbiology and biotechnology , strain (injury) , escherichia coli , biology , bacteria , chemistry , gene , vibrio , biochemistry , genetics , anatomy

N-Acylhomoserine lactones (AHLs) are used as quorum-sensing signal molecules by many gram-negative bacteria. We have reported that Shewanella sp. strain MIB015 degrades AHLs. In the present study, we cloned the aac gene from MIB015 by PCR with specific primers based on the aac gene in Shewanella oneidensis strain MR-1, which showed high homology with the known AHL-acylases. Escherichia coli expressing Aac showed high degrading activity of AHLs with long acyl chains. HPLC analysis revealed that Aac worked as AHL-acylase, which hydrolyzed the amide bond of AHL. In addition, expression of Aac in fish pathogen Vibrio anguillarum markedly reduced AHL production and biofilm formation. In conclusion, this study indicates that Aac might be effective in quenching quorum sensing of fish pathogens.

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